FIGURE

Fig. 4

ID

ZDB-FIG-090921-9

Publication

Nilsson et al., 2009 - Discovery of genes essential for heme biosynthesis through large-scale gene expression analysis

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Fig. 4

Morpholino Knockdown of Candidate Genes in Zebrafish Results in Profound Anemia
WT zebrafish embryos were injected at the one-cell stage with the respective morpholinos and stained at 48 hpf with o-dianisidine to detect hemoglobinized cells.
(A) Uninjected (WT) embryos show normal hemoglobinization as indicated by dark brown staining on the yolk sac (arrow).
(B–G) Morpholino-injected embryos. Accurate morpholino gene targeting was verified by RT-PCR (slc25a39, slc22a4, slc22a5, tmem14c, and c1orf69) or real-time quantitative RT-PCR (isca1) on cDNA from uninjected (WT) or morpholino-injected (mo) embryos. β-actin (actb) was used as a control for off-target effects in the RT-PCR. For RT-PCR, (ctrl) indicates no cDNA template control. For real-time quantitative RT-PCR, (ctrl mo) indicates embryos injected with a standard control morpholino.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-iba57 WT + MO1-isca1 WT + MO1-slc22a4 WT + MO1-slc22a5 WT + MO1-slc25a39 WT + MO1-tmem14ca
Knockdown Reagents:	MO1-iba57 MO1-isca1 MO1-slc22a4 MO1-slc22a5 MO1-slc25a39 MO1-tmem14ca
Observed In:	heme biosynthetic process nucleate erythrocyte nucleate erythrocyte hemoglobin complex
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Cell Metabolism, 10(2), Nilsson, R., Schultz, I.J., Pierce, E.L., Soltis, K.A., Naranuntarat, A., Ward, D.M., Baughman, J.M., Paradkar, P.N., Kingsley, P.D., Culotta, V.C., Kaplan, J., Palis, J., Paw, B.H., and Mootha, V.K., Discovery of genes essential for heme biosynthesis through large-scale gene expression analysis, 119-130, Copyright (2009) with permission from Elsevier. Full text @ Cell Metab.