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Fig. 5

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ZDB-FIG-090921-10

Publication

Nilsson et al., 2009 - Discovery of genes essential for heme biosynthesis through large-scale gene expression analysis

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Fig. 5

Mouse Slc25a39 Is Expressed in Hematopoietic Tissues and Is Important for Heme Biosynthesis
(A) Mouse tissue northern blot analysis of Slc25a39 expression.
(B) Mouse Slc25a39 transcripts are localized to blood islands of the yolk sac at early somite stages (E8.5, arrows).
(C and D) Slc25a39 transcripts accumulate most abundantly in the liver, where expression is heterogeneous (E12.5).
(E and F) MEL cells were differentiated for 2 days in media containing 1.5% DMSO prior to (E) transient transfection with myc-tagged Slc25a39 or (F) silencing of Slc25a39 (si-Slc25a39) and/or Slc25a37 (si-Slc25a37) using siRNA oligos. Assays were performed after 2 additional days of differentiation. si-NS indicates silencing using nonspecific control oligos.
(E) Representative western blot using anti-myc (α-myc-Slc25a39) and anti-Slc25a37 antibodies. Equal loading was verified by anti-tubulin.
(F) MEL cells were metabolically labeled with ⁵⁹Fe conjugated to transferrin, and total mitochondrial iron (⁵⁹Fe-Mito) and iron in heme (⁵⁹Fe-Heme) were determined. Results shown are from two independent experiments assayed in duplicate; error bars denote standard deviation.
(G–I) Representative photos of (G) an uninjected, wild-type control zebrafish embryo, (H) a zebrafish embryo injected with a ferrochelatase-specific morpholino, or (I) a zebrafish embryo injected with a slc25a39-specific morpholino. Arrow indicates porphyric red blood cells in circulation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-fech
Knockdown Reagent:	MO1-fech
Observed In:	nucleate erythrocyte hemoglobin complex
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Cell Metabolism, 10(2), Nilsson, R., Schultz, I.J., Pierce, E.L., Soltis, K.A., Naranuntarat, A., Ward, D.M., Baughman, J.M., Paradkar, P.N., Kingsley, P.D., Culotta, V.C., Kaplan, J., Palis, J., Paw, B.H., and Mootha, V.K., Discovery of genes essential for heme biosynthesis through large-scale gene expression analysis, 119-130, Copyright (2009) with permission from Elsevier. Full text @ Cell Metab.