ZFIN ID: ZDB-FIG-090904-26
Mei et al., 2009 - hnRNP I is required to generate the Ca2+ signal that causes egg activation in zebrafish. Development (Cambridge, England)   136(17):3007-3017 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: 1-cell

Fig. 3 Reduced Ca2+ wave causes egg activation defects in brom bones that is rescued by either Ca2+ or IP3. (A) Representative profiles of luminescence from aequorin-loaded zebrafish eggs (red: strongly affected brom bones mutant egg; blue: moderately affected mutant egg; green: mildly affected mutant egg). The profiles illustrate the total luminescent output for the entire activation process with a temporal resolution of 1 second. Arrow indicates the addition of 0.5% fructose in egg water to the egg-injection chamber to activate the eggs. Asterisks indicate the time window when the photon counting was suspended and a bright-field image of the egg was taken. Bright-field images show the egg morphology after activation, with the color of the box outline corresponding to the luminescence profile. Arrowheads indicate the degree of chorion elevation after activation. Scale bar: 100 μm. (B) Calcium injection rescues the egg activation defects in brom bones mutants. The degree of egg activation was examined after injection of KCl (control) or CaCl2 into brom bones eggs. brb1, brb2 and brb3 were three brom bones mutant females that produced eggs in this experiment. Eggs from brb2 and brb3 showed a similar range of phenotype and rescue, and were therefore combined for simplicity. The egg activation phenotype was scored as a percentage of the total number of eggs, with the strength of egg activation divided into five classes (shown in the bright-field images, right). (C-E)IP3 injection rescues the egg activation defects in brom bones mutants. (C,D) Eggs from a single clutch at 60 mpa after injection of buffer (C) or IP3 (D). (E) Percentages of eggs showing different degrees of egg activation after injection of buffer or IP3. brb1 and brb2 were two mutant females that produced eggs for this experiment and are different from the females in B.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ptbp1ap50ada standard conditions 1-cell egg activation disrupted, abnormal
1-cell positive regulation of cortical granule exocytosis by positive regulation of cytosolic calcium ion concentration disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development