ZFIN ID: ZDB-FIG-090904-25
Mei et al., 2009 - hnRNP I is required to generate the Ca2+ signal that causes egg activation in zebrafish. Development (Cambridge, England)   136(17):3007-3017 Full text @ Development
Observed In:
Stage: 1-cell

Fig. 2 CGE is defective in brom bones mutants. (A,B)F-actin distribution in wild-type (A) and brom bones mutant (B; 100%, n=28) zebrafish eggs prior to egg activation. (C,E) F-actin distribution in wild-type eggs at 2 (C) and 5 (E) mpa. (D,F) Abnormal F-actin distribution in brom bones mutant eggs at 2 (71%, n=35) and 5 (77%, n=49) mpa. The remaining mutant eggs showed F-actin distribution similar to wild-type eggs. Arrows in F highlight some of the circular profiles evident in mutant eggs. (G-L) F-actin (red) and CG content (green) distribution in wild-type (G,I,K) and brom bones mutant (H,J,L) eggs at 30 mpa (100%, n=71 brom bones mutant eggs). (M) Lateral view of a mutant egg surface at 5 mpa shows the localization of CG cores with respect to the F-actin meshwork. Arrow indicates a non-exocytosed granule within the F-actin meshwork; arrowheads indicate non-exocytosed granules beneath the F-actin meshwork. C-F are 20x magnification images; A-L are z-stacks of confocal images, M is a single confocal section. Scale bars: 10 μm in A,B; 20 μm in G-M.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ptbp1ap50ada standard conditions 1-cell cortical granule exocytosis disrupted, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development