ZFIN ID: ZDB-FIG-090904-25
Mei et al., 2009 - hnRNP I is required to generate the Ca2+ signal that causes egg activation in zebrafish. Development (Cambridge, England)   136(17):3007-3017 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: 1-cell

Fig. 2 CGE is defective in brom bones mutants. (A,B)F-actin distribution in wild-type (A) and brom bones mutant (B; 100%, n=28) zebrafish eggs prior to egg activation. (C,E) F-actin distribution in wild-type eggs at 2 (C) and 5 (E) mpa. (D,F) Abnormal F-actin distribution in brom bones mutant eggs at 2 (71%, n=35) and 5 (77%, n=49) mpa. The remaining mutant eggs showed F-actin distribution similar to wild-type eggs. Arrows in F highlight some of the circular profiles evident in mutant eggs. (G-L) F-actin (red) and CG content (green) distribution in wild-type (G,I,K) and brom bones mutant (H,J,L) eggs at 30 mpa (100%, n=71 brom bones mutant eggs). (M) Lateral view of a mutant egg surface at 5 mpa shows the localization of CG cores with respect to the F-actin meshwork. Arrow indicates a non-exocytosed granule within the F-actin meshwork; arrowheads indicate non-exocytosed granules beneath the F-actin meshwork. C-F are 20x magnification images; A-L are z-stacks of confocal images, M is a single confocal section. Scale bars: 10 μm in A,B; 20 μm in G-M.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ptbp1ap50ada standard conditions 1-cell cortical granule exocytosis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development