FIGURE

Fig. 3

ID

ZDB-FIG-090721-8

Publication

Ju et al., 2009 - Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish

Other Figures

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Fig. 3

Elevated phospho-AKT1 and Patched 1 levels in zebrafish tumors. A, B, a 3-week-old transgenic fish with trunk tumor (B) and its age-matched tumor-free fish (A). C, D, a 12-week-old fish with an eye tumor (D) and its age-matched tumor free fish (C). Immunofluorescence was done on cryosections for the above fish. E-H, an astrocytoma from a double transgenic fish showed elevated levels of both Patched 1 (F) and phosphorylated AKT (H). Immunofluoresence was done on paraffin sections for this tumor. Negative controls for Patched 1 (E) and phosphorylated AKT (G) were treated the same way except no primary antibodies were added. N, Notochord; AKT-P, phosphorylated AKT. Scale bars, 100 μm for A-D, 50 μm for E-H.

Expression Data

Gene:	ptch2
Antibodies:	Ab1-ptch2 Ab7-akt
Fish:	vu450Tg; vu452Tg
Anatomical Term:	trunk
Stage:	Days 21-29

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vu450Tg; vu452Tg
Observed In:	trunk astrocytoma
Stage:	Days 21-29

Phenotype Detail

Acknowledgments

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Open Access.

Full text @ Mol. Cancer