Fig. 5
Misexpression of mstn rescues axin1/apc1 embryos. (A) Injection of 5 pg p21CIP/WAF or mstn mRNA into 1 cell stage embryos, and phenotype assessment at 54 hpf. Slightly hypotrophic muscle fibers are observed in p21 as well as mstn-injected wild-type embryos confirming efficiency of misexpression. Muscle hypertrophy in mstn-injected axin1/apc1 embryos is partially restored to normal. (B) Quantification of the somite number of uninjected and p21CIP/WAF or mstn mRNA (5 pg) injected embryos. (C) Misexpression of mstn partially restores the truncated somite phenotype, as well as cell survival in axin1/apc1. Scale bar, 0.5 mm. |
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Stage: | Long-pec |