ZFIN ID: ZDB-FIG-090527-4
Vitorino et al., 2009 - Vsx2 in the zebrafish retina: restricted lineages through derepression. Neural Development   4:14 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4

Fig. 3 Vsx1 expression in the zebrafish retina. (A-D)vsx1 in situ hybridization in 24 hours post-fertilization (hpf) (A), 46 hpf (B), 62 hpf (C) and 80 hpf (D) Tg(vsx1:GFP) transgenic embryos shows complete co-localisation of vsx1 RNA and Vsx1 driven GFP expression. (E-I)Whole-mount lateral view of the Tg(vsx1:GFP) retina of zebrafish embryos counterstained with DAPI (blue) reveals expression of green fluorescent protein (GFP) in a subpopulation of retinal progenitor cells from 24 to 48 hpf as well as differentiated neurons at 72 and 96 hpf. (H-L)Cryosections through the central retina of Tg(vsx1:GFP) reveal GFP expression in cell bodies in the outer INL and processes in the OPL and IPL (arrows). GFP is also expressed in a few cells in the inner half of the INL (some indicated by asterisks). (J-L) Tg(vsx1:GFP) retinas at 80 hpf labelled with protein kinase C (PKC)β1 (J), calretinin (K) and Sox2 (L). Vsx1:GFP cells co-label with PKC-positive bipolar cells and calretinin-positive amacrine cells, but not with Sox2-labelled Müller cells. GCL, ganglion cell layer; INL, inner nuclear layer; ipl, inner plexiform layer; L, lens; ONL, outer nuclear layer; opl, outer plexiform layer. Scale bars: (A-I) 100 μm; (J-L) 40 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP nns5Tg standard conditions Prim-5 amacrine cell IFL
Prim-5 Not Detected Muller cell IFL
Prim-15 amacrine cell IFL
Prim-15 retinal inner nuclear layer IFL
High-pec retinal inner nuclear layer IFL
Long-pec retinal inner nuclear layer IFL
Pec-fin retinal inner nuclear layer IFL
Protruding-mouth retinal inner nuclear layer IFL
Day 4 Not Detected Muller cell IFL
sox2 Ab1-sox2 nns5Tg standard conditions Day 4 retinal inner nuclear layer Muller cell IHC
vsx1 nns5Tg standard conditions Prim-5 optic cup ISH
High-pec retinal inner nuclear layer ISH
Pec-fin retinal inner nuclear layer ISH
Protruding-mouth retinal inner nuclear layer ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-calb2 IHC nns5Tg standard conditions Protruding-mouth amacrine cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.