ZFIN ID: ZDB-FIG-090513-20
Trapani et al., 2009 - Synaptojanin1 is required for temporal fidelity of synaptic transmission in hair cells. PLoS Genetics   5(5):e1000480 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage Range: Protruding-mouth to Days 21-29
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Days 21-29

Fig. 3 Basal blebbing in synj1Q296X hair cells occurs near synaptic ribbons and is Ca2+-dependent.

FM 1–43 labeling of wild-type (A) and synj1Q296X (B) neuromast hair cells. Note the bleb or membrane protrusion at the base of a mutant cell (arrow). Single optical sections of membrane-targeted EGFP (Tg(brn3c:mgfp)) expressed in wild-type (C) and mutant (F) hair cells. D, G) Ribeye-b immunolabel (red staining) of the same neuromasts, indicating ribbons. E, H) Overlays of membrane-targeted EGFP and Ribeye-b label. Arrows in panel H indicate blebs near the ribbon in mutant hair cells. I) Basal blebbing was Ca2+-dependent. Blebbing occurred minimally in neuromast hair-cells of wild-type larvae (black bar). In contrast, blebbing was significantly increased in synj1Q296X mutant hair cells (grey bar). Blebbing was minimal in cav1.3R1250X mutant hair cells (blue bar), as well as cav1.3R1250X/synj1Q296X double mutant hair cells (blue stripe bar). Blebbing still occurred in synj1Q296X mutants that were transduction-deficient by morpholino-mediated cadherin23 knockdown (grey stripe bar). Numbers in parentheses above bars indicate number of hair cells examined. Scale bars, 3 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctbp2l IHC synj1tQ296X/tQ296X; s356tTg standard conditions Protruding-mouth - Days 21-29 neuromast hair cell
Phenotype Details
Fish Conditions Stage Phenotype
cacna1datc323d/tc323d standard conditions Protruding-mouth - Days 21-29 neuromast hair cell plasma membrane shape, normal
cacna1datc323d/tc323d; synj1tQ296X/tQ296X standard conditions Protruding-mouth - Days 21-29 neuromast hair cell plasma membrane shape, normal
synj1tQ296X/tQ296X standard conditions Protruding-mouth - Days 21-29 neuromast hair cell plasma membrane protruding, abnormal
synj1tQ296X/tQ296X + MO1-cdh23 standard conditions Protruding-mouth - Days 21-29 neuromast hair cell plasma membrane protruding, abnormal
synj1tQ296X/tQ296X; s356tTg standard conditions Protruding-mouth - Days 21-29 neuromast hair cell plasma membrane protruding, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.