FIGURE

Fig. S4b

ID

ZDB-FIG-090504-37

Publication

Kalén et al., 2009 - Combination of reverse and chemical genetic screens reveals angiogenesis inhibitors and targets

Other Figures

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Fig. S4b

Summary of Zebrafish Knock-Down Phenotypes The results from the MPO knock-down of (a) fzd6, (b) X99384, (c) ctsz, (d) rab11, (e) hexb, (f) p2ry5, (g) ralb, orthologue 1, (h) ralb, orthologue 2, (i) rab5c, (j) ppap2a, (k) ppp4c, (l) ppp1ca, (m) gnb2l1, (n) sat1, (o) ppp1cc, (p) ppih and (q) alox5ap, are listed with regards to:

The morphology at 28 and 56hpf of control and MPO knock-down zebrafish embryos.
The results from the cdh5 in situ hybridization at 48-56hpf.
The results from the FITC-dextran Microangiography at 56hpf.

The vascular defects were categorized as being Normal, Low (i.e. <30% absence of intersegmental vessel staining or FITC-Dextran signal), Medium (i.e. 31-70% defective intersegmental vessel staining/signal) or High (i.e., >70% defective intersegmental vessel staining/signal).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-alox5ap + MO2-alox5ap WT + MO1-plpp1b + MO2-plpp1b WT + MO1-ppih + MO2-ppih WT + MO1-ppp1cab + MO2-ppp1cab WT + MO1-ppp1cc + MO2-ppp1cc WT + MO1-ppp4ca + MO2-ppp4ca WT + MO1-rack1 + MO2-rack1 WT + MO1-sat1b + MO2-sat1b WT + MO2-rab5c + MO3-rab5c
Knockdown Reagents:	MO1-alox5ap MO1-plpp1b MO1-ppih MO1-ppp1cab MO1-ppp1cc MO1-ppp4ca MO1-rack1 MO1-sat1b MO2-alox5ap MO2-plpp1b MO2-ppih MO2-ppp1cab MO2-ppp1cc MO2-ppp4ca MO2-rab5c MO2-rack1 MO2-sat1b MO3-rab5c
Observed In:	blood circulation blood vasculature cell death extension eye fourth ventricle head hindbrain intersegmental vessel pericardium whole organism yolk
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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