FIGURE

Fig. S4a

ID

ZDB-FIG-090504-36

Publication

Kalén et al., 2009 - Combination of reverse and chemical genetic screens reveals angiogenesis inhibitors and targets

Other Figures

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Fig. S4a

Summary of Zebrafish Knock-Down Phenotypes The results from the MPO knock-down of (a) fzd6, (b) X99384, (c) ctsz, (d) rab11, (e) hexb, (f) p2ry5, (g) ralb, orthologue 1, (h) ralb, orthologue 2, (i) rab5c, (j) ppap2a, (k) ppp4c, (l) ppp1ca, (m) gnb2l1, (n) sat1, (o) ppp1cc, (p) ppih and (q) alox5ap, are listed with regards to:

The morphology at 28 and 56hpf of control and MPO knock-down zebrafish embryos.
The results from the cdh5 in situ hybridization at 48-56hpf.
The results from the FITC-dextran Microangiography at 56hpf.

The vascular defects were categorized as being Normal, Low (i.e. <30% absence of intersegmental vessel staining or FITC-Dextran signal), Medium (i.e. 31-70% defective intersegmental vessel staining/signal) or High (i.e., >70% defective intersegmental vessel staining/signal).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ctsz + MO2-ctsz WT + MO1-fzd6 + MO2-fzd6 WT + MO1-hexb + MO2-hexb WT + MO1-lpar6a + MO2-lpar6a WT + MO1-pald1a + MO2-pald1a WT + MO1-rab11a + MO2-rab11a WT + MO1-ralbb + MO2-ralbb
Knockdown Reagents:	MO1-ctsz MO1-fzd6 MO1-hexb MO1-lpar6a MO1-pald1a MO1-rab11a MO1-ralbb MO2-ctsz MO2-fzd6 MO2-hexb MO2-lpar6a MO2-pald1a MO2-rab11a MO2-ralbb
Observed In:	blood circulation blood vasculature cell death extension eye fourth ventricle head intersegmental vessel pericardium whole organism yolk
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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