FIGURE

Fig. S6

ID

ZDB-FIG-090504-16

Publication

Zeng et al., 2009 - Phospholipase D1 is required for angiogenesis of intersegmental blood vessels in zebrafish

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Fig. S6

Loss of Pld1 function disrupts the cranial vessels and blood cells formation. (A) At 48 hpf, Control TG(flk1:GFP)/(gata1:DsRed) embryos show a normal formation of ISV and axis vessels (green) in the trunk region (lateral view) with flowing blood cells in red, and 0.3% 1-BuOH treated embryos (B) show defective ISV formation and slightly reduced axis formation at the similar area, however blood cells are much more strongly affected. At 48 hpf, compared to control embryos (C), major cranial vessels in the head region are mainly formed in MO2-pld1 injected embryos (D), with minor disorganization of CCtA (Cerebellar central artery) (white arrows) and reduction of IOC vessels (Inner optic circle, white dotted circle) in the retinal area. At 12 hpf (6-somite stage), uninjected control embryos (E, group; F, dorsal view) express gata1 bilaterally in the lateral plate mesoderm, however the expression are strongly reduced or absent in MO2-pld1 injected embryos (G, group; H, dorsal view).

Expression Data

Gene:	gata1a
Fish:	WT WT + MO2-pld1a
Knockdown Reagent:	MO2-pld1a
Anatomical Term:	lateral plate mesoderm
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-pld1a zn1Tg + MO2-pld1a
Knockdown Reagent:	MO2-pld1a
Observed In:	cerebellar central artery erythrocyte differentiation inner optic circle nucleate erythrocyte vasculogenesis
Stage Range:	5-9 somites to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 328(2), Zeng, X.X., Zheng, X., Xiang, Y., Cho, H.P., Jessen, J.R., Zhong, T.P., Solnica-Krezel, L., and Brown, H.A., Phospholipase D1 is required for angiogenesis of intersegmental blood vessels in zebrafish, 363-376, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.