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ZFIN ID: ZDB-FIG-090501-43
Ohata et al., 2009 - Neuroepithelial cells require fucosylated glycans to guide the migration of vagus motor neuron progenitors in the developing zebrafish hindbrain. Development (Cambridge, England)   136(10):1653-1663 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 6 Confirmation of the gmds gene as the twdrw685 locus by knock-down and rescue experiments. (A) The efficacy of the splice-blocking MO against gmds was confirmed by RT-PCR at 32 hpf. Total RNA of gmds morphants and 5-mis-pair control morphants was incubated with (RT+) or without (RT–) reverse transcriptase. Samples were amplified with primers in which the target region of the MO is covered. Therefore, a band is not detected if maturation of gmds mRNA is inhibited by the MO. ef1α; loading control. (B) Dorsal views of the gmds morphant and twdrw685 mutant embryos injected with GMDS mRNAs at 50 hpf (rostral towards the top). Wild-type embryos injected with the 5-mis-pair control MO (a), the gmds MO (b) and S-GMDS mRNA (e). The twdrw685 mutants injected with L-gmds mRNA (c,c′) and S-gmds mRNA (d). Completely rescued (c,d) and partially rescued (c′) mutants. Arrowheads indicate ectopic vagus motor neurons. (C) Time-lapse observation of the migration of vagus motor neuron progenitors in twdrw685 embryos injected with L-gmds mRNA. The broken line shows the outline of the hindbrain. Dorsal is towards the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP rw0Tg standard conditions Long-pec facial nerve motor nucleus IFL
Long-pec hindbrain nucleus IFL
Long-pec trigeminal motor nucleus IFL
Long-pec vagal lobe IFL
rw0Tg + MO1-gmds standard conditions Long-pec facial nerve motor nucleus IFL
Long-pec hindbrain nucleus IFL
Long-pec trigeminal motor nucleus IFL
Long-pec vagal lobe IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rw0Tg + MO1-gmds standard conditions Long-pec dorsolateral motor nucleus of vagal nerve fused with dorsolateral motor nucleus of vagal nerve, abnormal
Long-pec dorsolateral motor nucleus of vagal nerve mislocalised medially, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development