FIGURE

Fig. 6

ID

ZDB-FIG-090401-14

Publication

Carvalho et al., 2009 - Control of convergent yolk syncytial layer nuclear movement in zebrafish

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Fig. 6

Filamentous actin within the YSL is required for convergence movement coordination between iYSN and mesendoderm. (A,B). Actin labeled with Lifeact-GFP in embryos incubated in cytochalasin (A) and injected with Phalloidin into the YSL (B) at 90% epiboly (9 hpf; A) and bud stage (10 hpf; B). Asterisks mark iYSN. (C,D) Trajectories of iYSN (blue tracks) and mesendoderm progenitors (orange tracks) in cytochalasin-treated (C) and phalloidin YSL-injected (D) embryos at mid-gastrulation stages (7-8 hpf). Images are z-projections. Dorsal is towards the right. (E,F) Quantification of the similarity between iYSN and mesendoderm convergence movements in cytochalasin-treated (E) and phalloidin YSL-injected (F) embryos at mid-gastrulation stages (7-8 hpf). Histograms of the similarity values were generated separately for each embryo. Box plots show the distribution of the bin heights among the embryos. (E) In cytochalasin-treated embryos, 34% of the similarity values are higher than 0.5 (n=3 embryos). (F) In phalloidin YSL-injected embryos, 41% of the similarity values are higher than 0.5 (n=3 embryos). mes, mesendoderm progenitor. Scale bars: 2 μm in A; 20 μm in B; 50 μm in C,D.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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