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Asymmetric cardiac jogging results in a L/R to D/V axis conversion. Shown are dorsal views of lefty2 expression in the heart at 20 hpf and 24–26 hpf (A, B, E, and F) and in transverse histological sections at 24–26 hpf with heart tubes outlined in black (C, D, G, and H). Sections in D and H have been stained with hematoxylin and eosin. In WT, lefty2 expression changes from left restricted expression at 20 hpf (A) to a symmetric extension at 24–26 hpf (B). Sections reveal that lefty2 localization is restricted to the dorsal side of the heart (C and D). swt morphants that likely express lefty2 on the right at 20 hpf (E) consistently display reversed cardiac jogging but also exhibit symmetric expression of lefty2 along the length of the heart tube (F). However, sections reveal a dorsal localization of lefty2 RNA, similar to WT (G and H). D, dorsal; V, ventral.
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