FIGURE

Fig. 3

ID

ZDB-FIG-090212-1

Publication

Jones et al., 2008 - A High Throughput Live Transparent Animal Bioassay to Identify Non-toxic Small Molecules or Genes that Regulate Vertebrate Fat Metabolism for Obesity Drug Development

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Fig. 3

Zebrafish respond to mammalian regulators of fat metabolism in an evolutionarily conserved manner. Similar to pharmacological application of niacin in the clinic, nicotinic acid inhibits zebrafish lipolysis (A), decreases total cholesterol (B), and increases expression of the most potent quantitative scavenger of oxidized lipids, CD36 and the classic marker of adipocyte differentiation, FABP4 (D). Zebrafish larvae were exposed to nicotinic acid (NA), nicotinamide (NAM), resveratrol (R), or resveratrol with norepinephrine (NE) from 3–7 dpf before extraction of total phospholipids for quantitation by gas chromatography (A) or quantitation of transcript levels (C and D). For cholesterol measurements zebrafish were treated with 1 mM concentrations of NA or NAM from 3–10 dpf (B).

Expression Data

Genes:	cd36 fabp4b gpr109al
Fish:	WT
Conditions:	chemical treatment: 15-deoxy-Delta(12,14)-prostaglandin J2 chemical treatment: nicotinamide chemical treatment: nicotinic acid hypoxia chemical treatment: nicotinic acid, hypoxia
Anatomical Term:	whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Open Access.

Full text @ Nutr. Metab. (Lond).