FIGURE

Fig. 6

ID

ZDB-FIG-090127-25

Publication

Targoff et al., 2008 - Nkx genes regulate heart tube extension and exert differential effects on ventricular and atrial cell number

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Fig. 6

nkx genes limit atrial cell number during heart tube assembly. (A,B) Immunofluorescence indicates nuclear localization of Mef2 (green) throughout the heart tube, facilitating counting of differentiated cardiomyocytes at 26 hpf. Atrial cells are indicated by the anti-Amhc antibody, S46 (red). Hearts are flattened with a cover slip to improve visualization of cardiomyocyte nuclei. (C) Quantification of cardiomyocyte nuclei in wild-type (n = 25) and nkx-deficient (n = 11) embryos reveals a statistically significant increase in atrial cell number in nkx-deficient embryos (p < 0.001, Student's t-test) and no significant difference in ventricular cell number. Bar graph indicates mean and standard error of each data set; asterisk indicates statistically significant difference from wild-type.

Expression Data

Antibodies:	Ab-S46 Ab1-mef2
Fish:	WT WT + MO1-nkx2.7 + MO2-nkx2.5
Knockdown Reagents:	MO1-nkx2.7 MO2-nkx2.5
Anatomical Term:	heart tube cardiac muscle cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-nkx2.7 + MO2-nkx2.5
Knockdown Reagents:	MO1-nkx2.7 MO2-nkx2.5
Observed In:	atrial myocardium cardiac muscle cell embryonic heart tube development heart tube ventricular myocardium cardiac muscle cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 322(2), Targoff, K.L., Schell, T., and Yelon, D., Nkx genes regulate heart tube extension and exert differential effects on ventricular and atrial cell number, 314-321, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.