FIGURE

Fig. 3

ID

ZDB-FIG-090106-5

Publication

Zhou et al., 2008 - Duplication of the IGFBP-2 gene in teleost fish: protein structure and functionality conservation and gene expression divergence

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Fig. 3

Zebrafish igfbp-2b encodes a secreted protein that binds IGFs.

Conditioned media was prepared from HEK293T cells transfected with pCMV-human IGFBP-2-Myc (hIGFBP-2) or pCMV-zebrafish IGFBP-2b-Myc plasmids. The conditioned media were analyzed by Western immunoblotting (WB) using c-Myc antibody (left panel) and ligand blotting using DIG-labeled human IGF-1 (right panel).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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