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Fig. S7

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ZDB-FIG-081222-37

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Waxman et al., 2008 - Hoxb5b acts downstream of retinoic Acid signaling in the forelimb field to restrict heart field potential in zebrafish

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Fig. S7

Activation of the Transgene Tg(hsp70:dnRARα) Causes Phenotypes Consistent with a Loss of RA Signaling
(A–D) Heat shock of embryos carrying the transgene Tg(hsp70:dnRARα) causes pericardial edema (arrowhead in C), shortened hindbrain (arrow in C), and loss of pectoral fins (outlined in B); lateral views, anterior to the left, of live embryos at 48 hpf, with higher magnification of pectoral fin region shown in (B,D).
(E and H) tbx5 expression at 30 hpf; dorsal views, anterior to the top. In heat shocked transgenic embryos, tbx5 expression is lost from the forelimb region.
(F and I) cmlc2 expression at 22 hpf; dorsal views, anterior to the top. Heat shocked transgenic embryos display a modest expansion of cmlc2 expression.
(G and J) At 48 hpf, hearts of heat-shocked transgenic embryos are modestly dilated and exhibit an increase in atrial cell number; see also Table S1. Images and cell counts were generated by crossing a fish carrying the transgene Tg(hsp70:dnRARα) with a fish carrying the transgene Tg(cmlc2:DsRed2-nuc) . Both non-heat shocked, transgenic siblings and non-transgenic, heat shocked siblings were morphologically normal and considered wild-type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 15(6), Waxman, J.S., Keegan, B.R., Roberts, R.W., Poss, K.D., and Yelon, D., Hoxb5b acts downstream of retinoic Acid signaling in the forelimb field to restrict heart field potential in zebrafish, 923-934, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell