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Fig. S1

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ZDB-FIG-081222-31

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Waxman et al., 2008 - Hoxb5b acts downstream of retinoic Acid signaling in the forelimb field to restrict heart field potential in zebrafish

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Fig. S1

The Numbers of atrial and Ventricular Cells Are Increased in raldh2 Morphants and neckless (nls/raldh2) Mutants
All views and graphs are as presented in Figure 1.
(A and B, F and G) raldh2 morphants exhibit increased atrial and ventricular cell number; see also Table S3. Asterisks indicate statistically significant differences relative to wild-type (p<0.01).
(C–E and H–J) nls mutants have increased expression of amhc and vmhc; see also Supplemental Table 4. Asterisks indicate statistically significant differences relative to wild-type (p<0.005). The phenotypes of raldh2 morphants and nls mutants are less dramatic than those observed in BMS-treated embryos (Figure 1), most likely because of limits on MO efficacy and the hypomorphic nature of the nls allele (Begemann et al., 2001).

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Cell, 15(6), Waxman, J.S., Keegan, B.R., Roberts, R.W., Poss, K.D., and Yelon, D., Hoxb5b acts downstream of retinoic Acid signaling in the forelimb field to restrict heart field potential in zebrafish, 923-934, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell