Fig. S7

Fig. S7 Activation of the Transgene Tg(hsp70:dnRARα) Causes Phenotypes Consistent with a Loss of RA Signaling
(A–D) Heat shock of embryos carrying the transgene Tg(hsp70:dnRARα) causes pericardial edema (arrowhead in C), shortened hindbrain (arrow in C), and loss of pectoral fins (outlined in B); lateral views, anterior to the left, of live embryos at 48 hpf, with higher magnification of pectoral fin region shown in (B,D).
(E and H) tbx5 expression at 30 hpf; dorsal views, anterior to the top. In heat shocked transgenic embryos, tbx5 expression is lost from the forelimb region.
(F and I) cmlc2 expression at 22 hpf; dorsal views, anterior to the top. Heat shocked transgenic embryos display a modest expansion of cmlc2 expression.
(G and J) At 48 hpf, hearts of heat-shocked transgenic embryos are modestly dilated and exhibit an increase in atrial cell number; see also Table S1. Images and cell counts were generated by crossing a fish carrying the transgene Tg(hsp70:dnRARα) with a fish carrying the transgene Tg(cmlc2:DsRed2-nuc) . Both non-heat shocked, transgenic siblings and non-transgenic, heat shocked siblings were morphologically normal and considered wild-type.