FIGURE

Fig. 1

ID

ZDB-FIG-081125-3

Publication

Morton et al., 2008 - microRNA-138 modulates cardiac patterning during embryonic development

Other Figures

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Fig. 1

miR-138 is required for cardiac development. (A) Oblique view of 72 hpf fish after in situ hybridization showing expression of miR-138 in the ventricular chamber marked by blue staining. (B) qRT-PCR analysis of mature miR-138 expression at 48 hpf in flow-sorted cardiomyocytes (cmlc2:GFP), endothelial cells (flk1:GFP), or whole embryos demonstrated enrichment of miR-138 in cardiomyocytes. Mature miR-138 was not detected in endothelial cells. (C and D) Fish embryos injected with miR-138 scrambled control morpholino (scr) (C) or miR-138 morpholino (mo-1) (D) showing pericardial edema (asterisk); arrowhead indicates heart. (E–I) Representative confocal images of transgenic Tg(myl7:HRAS-mEGFP)^s843 uninjected embryos (E) or embryos injected with 17-nt miR-138^scr (F), 17-nt miR-138^mo-1 (G), or a second 31-nt miR-138^mo-2 oligo (H). Insets show single z-stack images of outer curvature ventricular myocytes, which normally elongate with maturation (E and F) but remained rounded in the miR-138^mo embryos (G and H). (e, eye; y, yolk sac; a, atrium; v, ventricle; avc, atrioventricular canal.)

Expression Data

Genes:	dre-mir-138 EGFP
Fish:	AB s843Tg s883Tg
Anatomical Terms:	cardiac muscle cell cardiac ventricle muscle cell
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-dre-mir-138 AB + MO2-dre-mir-138 s883Tg + MO1-dre-mir-138
Knockdown Reagents:	MO1-dre-mir-138 MO2-dre-mir-138
Observed In:	cardiac ventricle cardiac muscle cell heart looping pericardium
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Open Access.

Full text @ Proc. Natl. Acad. Sci. USA