FIGURE

Fig. S5

ID

ZDB-FIG-081125-11

Publication

Morton et al., 2008 - microRNA-138 modulates cardiac patterning during embryonic development

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Fig. S5

(A) Quantification of Raldh2-positive area in multiple immunohistochemistry sections as represented in main text Fig. 4 C and D. Results shown are the average of 4 slides per group. (B and C) Immunohistochemistry for Raldh2 protein in miR-138^am fish demonstrates Raldh2 expression (red) overlapping with Tg(flk1-EGFP)^s843 (B) in the atrioventricular canal (avc), and Raldh2 overlapping with Tg(cmlc2-ras-EGFP)^s843 in the ventricle (v) (C). cmlc2-ras-EGFP expression is localized to the cell membrane of cardiomyocytes. (D and E) In situ hybridization for aldh1a2 demonstrates AVC-restricted expression in control fish (D) but expanded ventricular expression in miR-138^mo fish (E). wt, wild-type; pa, pharyngeal arch; a, atrium.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Full text @ Proc. Natl. Acad. Sci. USA