ZFIN ID: ZDB-FIG-081107-38
Davuluri et al., 2008 - Mutation of the zebrafish nucleoporin elys sensitizes tissue progenitors to replication stress. PLoS Genetics   4(10):e1000240 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Conditions:
Anatomical Term:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 6 The flo mutation activates the DNA damage response.

(A–C) Acridine orange staining showing apoptosis in the 50 hpf flo retina that is rescued by the tp53 morpholino (mo) knockdown. (D) Histological cross section showing rescue of flo retinal architecture defects by tp53 knockdown [compare (F) with Figure 1G and 1H]. (E) Intestinal defects persist in flo/tp53 double mutants. Arrow, thin intestinal wall; Arrowhead, apoptotic cells in the intestinal lumen. (F) Western blot showing elevated levels of phospho-Chk2 (Serine 33) in the intestine of flo larvae, compared with sibling wild type larvae, but not slim jim larvae (I). (G) Western blot showing comparable levels of phospho-Chk1 (Ser 345) in flo and sibling wild type larvae, before and after γ-radiation (30 Gy) and treatment with hydroxyurea (HU). (H) Western blot showing enhanced phospho-Chk2 activation in the intestine of flo and wild type larvae following γ-radiation (30 Gy). (I) γH2AX is not detected in the flo or wild type intestine (75 hpf), but is detected at this stage following γ-irradiation (30 Gy) or hydroxyurea treatment (HU).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-chek1 WB WT standard conditions Protruding-mouth intestine
WB Day 4 intestine
WB WT chemical treatment: hydroxyurea Protruding-mouth intestine
WB WT radiation Protruding-mouth intestine
WB ahctf1ti262c/ti262c standard conditions Protruding-mouth intestine
WB Day 4 intestine
WB ahctf1ti262c/ti262c chemical treatment: hydroxyurea Protruding-mouth intestine
WB ahctf1ti262c/ti262c radiation Protruding-mouth intestine
Ab2-chek2 WB WT standard conditions Protruding-mouth intestine
WB WT chemical treatment: hydroxyurea Protruding-mouth intestine
WB WT radiation Protruding-mouth intestine
WB ahctf1ti262c/ti262c standard conditions Protruding-mouth intestine
WB ahctf1ti262c/ti262c chemical treatment: hydroxyurea Protruding-mouth intestine
WB ahctf1ti262c/ti262c radiation Protruding-mouth intestine
Ab2-h2afx WB WT standard conditions Protruding-mouth Not Detected intestine
WB WT chemical treatment: hydroxyurea Protruding-mouth intestine
WB WT radiation Protruding-mouth intestine
WB ahctf1ti262c/ti262c standard conditions Protruding-mouth Not Detected intestine
WB ahctf1ti262c/ti262c chemical treatment: hydroxyurea Protruding-mouth intestine
WB ahctf1ti262c/ti262c radiation Protruding-mouth intestine
Ab3-chek2 WB WT standard conditions Protruding-mouth intestine
WB Day 4 intestine
WB WT radiation Protruding-mouth intestine
WB ahctf1ti262c/ti262c standard conditions Protruding-mouth intestine
WB Day 4 intestine
WB ahctf1ti262c/ti262c radiation Protruding-mouth intestine
WB polr3bm74/m74 standard conditions Day 4 intestine
Phenotype Details
Fish Conditions Stage Phenotype
ahctf1ti262c/ti262c standard conditions Long-pec retina apoptotic, abnormal
ahctf1ti262c/ti262c + MO3-tp53 + MO4-tp53 standard conditions Long-pec retina structure, normal
Day 5 retina morphology, normal
ahctf1ti262c/ti262c; tp53zdf1/zdf1 standard conditions Day 4 intestine apoptotic, abnormal
Day 4 intestine morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.