ZFIN ID: ZDB-FIG-081103-8
Aamar et al., 2008 - Isolation and expression analysis of foxj1 and foxj1.2 in zebrafish embryos. The International journal of developmental biology   52(7):985-991 Full text @ Int. J. Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Protruding-mouth

Fig. 4 Zebrafish foxj1.2 expression. (A-P) Spatio-temporal expression pattern of foxj1.2. Whole-mount in situ hybridization with foxj1.2 probe alone (A-D, F-G, L, M4), or combined with pax2.1 and charon (both red) (E, H-K, M-P). Shield-60% epiboly (A), 80% epiboly (B), bud (C-E), 4 somites (FG), 7 somites (H-J), 15 somites (K,P), 16 somites (L), 1 day (M), 2 days (N), 3 and 4 days (P left and right respectively). Views are as follows: dorsal (A, B); dorsal with anterior to the left (C bottom, E, F bottom, H and H bottom), dorsal with anterior up (P right), lateral with dorsal to the right, (A left bottom, B left bottom), lateral with anterior to left (C top, F, I, K-O except for M4, P left), posterior with dorsal up (D, G, J), anterior ventral with dorsal up (M4), animal view (A left top, B left top), ventral with posterior to the right (J right bottom). (Q) RT-PCR expression analysis of foxj1.2 and β-actin as control was performed for different stages (1-8: unfertilized eggs, 100-200 cells, high-dome, 40-50% epiboly, 80-90%, bud, 13-somites, 24hpf and 3days old embryos respectively, and –RT in lane 10). d, diencephalon; dt, dorsal tectum; e, eye; fp, floor plate; k, kidney; KV, Kupffer’s vesicle; MHB, mid-hindbrain boundary; nt, notochord; op, olfactory pit; ot; otolith; ov, otic vesicle; p, pineal gland; pd, pronephric duct; pnt, posterior neural tube; t, tectum; tg, trigeminal ganglion; tv, tectal ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxj1b WT standard conditions 1-cell to Dome Not Detected whole organism RTPCR
Shield epiblast ISH
50%-epiboly to Protruding-mouth whole organism RTPCR
75%-epiboly epiblast ISH
1-4 somites otic placode ISH
1-4 somites posterior neural keel ISH
Bud presumptive floor plate ISH
1-4 somites presumptive floor plate ISH
Bud presumptive pronephric mesoderm ISH
1-4 somites pronephric mesoderm ISH
5-9 somites otic placode ISH
5-9 somites posterior neural keel ISH
5-9 somites pronephric mesoderm ISH
14-19 somites diencephalon ISH
14-19 somites floor plate ISH
14-19 somites olfactory placode ISH
14-19 somites otic vesicle ISH
14-19 somites trigeminal placode ISH
Prim-5 diencephalon ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 olfactory placode ISH
Prim-5 optic tectum ISH
Prim-5 otolith ISH
Long-pec epiphysis ISH
Long-pec olfactory pit ISH
Protruding-mouth tectal ventricle ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal The International journal of developmental biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Dev. Biol.