ZFIN ID: ZDB-FIG-081103-8 |
Gene: | |
---|---|
Fish: | |
Anatomical Terms: | |
Stage Range: | 1-cell to Protruding-mouth |
Fig. 4 Zebrafish foxj1.2 expression. (A-P) Spatio-temporal expression pattern of foxj1.2. Whole-mount in situ hybridization with foxj1.2 probe alone (A-D, F-G, L, M4), or combined with pax2.1 and charon (both red) (E, H-K, M-P). Shield-60% epiboly (A), 80% epiboly (B), bud (C-E), 4 somites (FG), 7 somites (H-J), 15 somites (K,P), 16 somites (L), 1 day (M), 2 days (N), 3 and 4 days (P left and right respectively). Views are as follows: dorsal (A, B); dorsal with anterior to the left (C bottom, E, F bottom, H and H bottom), dorsal with anterior up (P right), lateral with dorsal to the right, (A left bottom, B left bottom), lateral with anterior to left (C top, F, I, K-O except for M4, P left), posterior with dorsal up (D, G, J), anterior ventral with dorsal up (M4), animal view (A left top, B left top), ventral with posterior to the right (J right bottom). (Q) RT-PCR expression analysis of foxj1.2 and β-actin as control was performed for different stages (1-8: unfertilized eggs, 100-200 cells, high-dome, 40-50% epiboly, 80-90%, bud, 13-somites, 24hpf and 3days old embryos respectively, and –RT in lane 10). d, diencephalon; dt, dorsal tectum; e, eye; fp, floor plate; k, kidney; KV, Kupffer’s vesicle; MHB, mid-hindbrain boundary; nt, notochord; op, olfactory pit; ot; otolith; ov, otic vesicle; p, pineal gland; pd, pronephric duct; pnt, posterior neural tube; t, tectum; tg, trigeminal ganglion; tv, tectal ventricle. |
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
---|---|---|---|---|---|---|---|
foxj1b | WT | standard conditions | 1-cell to Dome | Not Detected | whole organism | RTPCR | |
Shield | epiblast | ISH | |||||
50%-epiboly to Protruding-mouth | whole organism | RTPCR | |||||
75%-epiboly | epiblast | ISH | |||||
1-4 somites | otic placode | ISH | |||||
1-4 somites | posterior neural keel | ISH | |||||
Bud | presumptive floor plate | ISH | |||||
1-4 somites | presumptive floor plate | ISH | |||||
Bud | presumptive pronephric mesoderm | ISH | |||||
1-4 somites | pronephric mesoderm | ISH | |||||
5-9 somites | otic placode | ISH | |||||
5-9 somites | posterior neural keel | ISH | |||||
5-9 somites | pronephric mesoderm | ISH | |||||
14-19 somites | diencephalon | ISH | |||||
14-19 somites | floor plate | ISH | |||||
14-19 somites | olfactory placode | ISH | |||||
14-19 somites | otic vesicle | ISH | |||||
14-19 somites | trigeminal placode | ISH | |||||
Prim-5 | diencephalon | ISH | |||||
Prim-5 | midbrain hindbrain boundary | ISH | |||||
Prim-5 | olfactory placode | ISH | |||||
Prim-5 | optic tectum | ISH | |||||
Prim-5 | otolith | ISH | |||||
Long-pec | epiphysis | ISH | |||||
Long-pec | olfactory pit | ISH | |||||
Protruding-mouth | tectal ventricle | ISH |
Acknowledgments: | ZFIN wishes to thank the journal The International journal of developmental biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Dev. Biol. |
---|