FIGURE

Fig. 1

ID

ZDB-FIG-081103-26

Publication

Thomas et al., 2008 - Hedgehog signaling plays a cell-autonomous role in maximizing cardiac developmental potential

Other Figures

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Fig. 1

Embryos with reduced Hedgehog signaling have small cardiac chambers. (A-D) Lateral views of zebrafish hearts stained with MF20 (red) and S46 (green) antibodies to visualize the ventricle and atrium at 48 hpf. MF20 marks the entire heart and S46 is atrium specific. In these superimposed images, the MF20⁺S46⁺ atrial tissue appears green and the MF20⁺S46^- ventricle is red. Scale bar: 100 μm. (A) Wild-type heart. (B-D) Zygotic smo^b577 mutant, MZsmo^b577 mutant and CyA-treated hearts have small, misshapen ventricles and atria. (E) Quantification of cardiomyocyte number in wild-type, smo^b577, MZsmo^b577 and CyA-treated embryos at 52 hpf. See Materials and methods for cell counting technique. Values represent the mean cell number for each category (±s.d.). Asterisks indicate statistically significant differences from wild type (P<0.0001 for all except the smo^b577 atrium where P=0.01).

Expression Data

Antibodies:	Ab-MF20 Ab-S46
Fish:	WT smo^b577/b577
Condition:	chemical treatment: Cyclopamine
Anatomical Terms:	atrium heart
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smo^b577/b577 smo^b577/b577 smo^b577/b577; f2Tg smo^b577/b577; f2Tg/+
Observed In:	atrium cardiac muscle cell cardiac ventricle cardiac muscle cell heart heart cardiac muscle cell
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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