ZFIN ID: ZDB-FIG-080925-6
Del Bene et al., 2008 - Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient. Cell   134(6):1055-1065 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 6 Atoh7 Loss of Function and Notch Activation Can Rescue Late Cell Fates in moks309

(A–D) Compared to wild-type (A), mok mutants (B) have an excess of RGCs and few bipolar cells, labeled by PKCα immunoreactivity. lak (atoh7) mutants fail to generate RGCs and overproduce bipolar neurons (C). lak/mok double mutants have rescued bipolar cell production.

(E–H) Heat-shocked induction of an activated form of Notch receptor NICD at 32 hpf drives progenitors toward a Müller glia fate (GS positive) in both wild-type (G) and moks309 retinas (H). The presence of the transgenes (hsp:Gal4, UAS:NICD) has no effect on Müller glia differentiation in the absence of heat shock (E and F).

Scale bars, 100 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-glul IHC dctn1as309/s309; kca3Tg; kca4Tg standard conditions Day 5 Muller cell
IHC dctn1as309/s309; kca3Tg; kca4Tg heat shock Day 5 Muller cell
IHC kca3Tg; kca4Tg standard conditions Day 5 Muller cell
IHC kca3Tg; kca4Tg heat shock Day 5 Muller cell
Ab1-prkca IHC TL standard conditions Day 5 retina
IHC atoh7th241/th241 standard conditions Day 5 retina
IHC atoh7th241/th241; dctn1as309/s309 standard conditions Day 5 retina
IHC dctn1as309/s309 standard conditions Day 5 retina
Phenotype Details
Fish Conditions Stage Phenotype
atoh7th241/th241 standard conditions Day 5 retinal bipolar neuron increased amount, abnormal
Day 5 retinal photoreceptor layer photoreceptor cell absent, abnormal
atoh7th241/th241; dctn1as309/s309 standard conditions Day 5 retinal bipolar neuron normal amount, normal
dctn1as309/s309 standard conditions Day 5 retinal bipolar neuron decreased amount, abnormal
Day 5 retinal ganglion cell increased amount, abnormal
dctn1as309/s309; kca3Tg; kca4Tg standard conditions Day 5 Muller cell increased amount, abnormal
dctn1as309/s309; kca3Tg; kca4Tg heat shock Day 5 Muller cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell