|ZFIN ID: ZDB-FIG-080925-4|
|Stage Range:||1-cell to Day 4|
Fig. 4 Expression of Dnct1 and Its Function in INM
(A) Western blotting of extracts from 4 dpf embryos shows that Dnct1 is undetectable in moks309.
(B) Time course analysis of Dnct1 expression by western blot in wild-type zebrafish (numbers on top indicate hours after fertilization).
(C and D) Whole-mount in situ hybridization shows dnct1 enriched in the head and eye region (C) and in the notochord (D). Scale bars, 100 μm.
(E and F) Coronal sections of 2 dpf retinas stained for the mitotic marker PH3. In moks309 (F), a number of mitotic cells are sparsely located throughout the retina, while, in the wild-type (E), they are confined within 2–3 cell diameters from the ventricular surface. Dashed lines demarcate the apical (right) and basal (left) domains. Asterisks indicate mitotic cells residing in the developing lens.
(G) Scatter plot of wild-type (gray triangles) and moks309 mutant (black squares) circles, showing the maximum basal distance of nuclei during INM from 30–48 hpf.
(H) Histogram showing that these populations are statistically different (p = 0.001, Wilcoxon two-sample test). Error bar indicates SEM.
|dctn1a||TL||standard conditions||1-cell to Day 4||whole organism||WB|
|Protruding-mouth||central nervous system||ISH|
|Protruding-mouth||retinal inner nuclear layer||ISH|
|dctn1as309/s309(TL)||standard conditions||Day 4||Not Detected||whole organism||WB|
|dctn1as309/s309(TL)||standard conditions||Long-pec||nuclear migration disrupted, abnormal|
|Long-pec||retina nucleus mislocalised, abnormal|
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell