ZFIN ID: ZDB-FIG-080925-4
Del Bene et al., 2008 - Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient. Cell   134(6):1055-1065 Full text @ Cell
Anatomical Terms:
Stage Range: 1-cell to Day 4
Observed In:
Stage: Long-pec

Fig. 4 Expression of Dnct1 and Its Function in INM

(A) Western blotting of extracts from 4 dpf embryos shows that Dnct1 is undetectable in moks309.

(B) Time course analysis of Dnct1 expression by western blot in wild-type zebrafish (numbers on top indicate hours after fertilization).

(C and D) Whole-mount in situ hybridization shows dnct1 enriched in the head and eye region (C) and in the notochord (D). Scale bars, 100 μm.

(E and F) Coronal sections of 2 dpf retinas stained for the mitotic marker PH3. In moks309 (F), a number of mitotic cells are sparsely located throughout the retina, while, in the wild-type (E), they are confined within 2–3 cell diameters from the ventricular surface. Dashed lines demarcate the apical (right) and basal (left) domains. Asterisks indicate mitotic cells residing in the developing lens.

(G) Scatter plot of wild-type (gray triangles) and moks309 mutant (black squares) circles, showing the maximum basal distance of nuclei during INM from 30–48 hpf.

(H) Histogram showing that these populations are statistically different (p = 0.001, Wilcoxon two-sample test). Error bar indicates SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dctn1a TL standard conditions 1-cell to Day 4 whole organism WB
Protruding-mouth central nervous system ISH
Protruding-mouth eye ISH
Protruding-mouth head ISH
Protruding-mouth retinal ganglion cell layer retinal ganglion cell ISH
Protruding-mouth retinal inner nuclear layer ISH
dctn1as309/s309(TL) standard conditions Day 4 Not Detected whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dctn1as309/s309(TL) standard conditions Long-pec nuclear migration disrupted, abnormal
Long-pec retina nucleus mislocalised, abnormal
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Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell