ZFIN ID: ZDB-FIG-080925-2
Del Bene et al., 2008 - Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient. Cell   134(6):1055-1065 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 2 moks309 Retinas Have Increased RGCs and Decreased Bipolar and Müller Glia Cells Due to Premature Neurogenesis

(A–H) Horizontal 5 dpf retina sections. GFP expression under the control of atoh7 (A and E) and Brn3c (Pou4f3) (B and F) promoters reveals an increase in RGCs in moks309 (E and F) as compared to the wild-type (A and B), with some RGCs ectopically located outside the GCL (yellow arrowheads in [E] and [F]). The Müller glia marker GS (C and G) and the bipolar cell marker PKCα (D and H) show fewer immunoreactive cell bodies (yellow arrowheads) in moks309 (G and H) as compared to wild-type (C and D).

(I–P) Horizontal 50 hpf retina sections stained for IdU (green, injected at 26 hpf), BrdU (red, injected at 38 hpf), and DAPI (blue). moks309 retinas (M–P) show more IdU-positive and BrdU-negative cells than wild-type, indicating that a larger number of progenitors had exited the cell cycle between 26 and 38 hpf.

Scale bars, 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP dctn1as309/s309; rw021Tg standard conditions Day 5 retinal ganglion cell layer retinal ganglion cell IFL
dctn1as309/s309; s356tTg standard conditions Day 5 retinal ganglion cell layer retinal ganglion cell IFL
rw021Tg standard conditions Day 5 retinal ganglion cell layer retinal ganglion cell IFL
s356tTg standard conditions Day 5 retinal ganglion cell layer retinal ganglion cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-BrdU/IdU IHC TL chemical treatment: 5-bromo-2'-deoxyuridine, chemical treatment: pharmaceutical Long-pec retina
IHC dctn1as309/s309 chemical treatment: 5-bromo-2'-deoxyuridine, chemical treatment: pharmaceutical Long-pec retina
Ab1-glul IHC TL standard conditions Day 5 retinal inner nuclear layer Muller cell
IHC dctn1as309/s309 standard conditions Day 5 retinal inner nuclear layer Muller cell
Ab1-prkca IHC TL standard conditions Day 5 retina retinal bipolar neuron
IHC dctn1as309/s309 standard conditions Day 5 retina retinal bipolar neuron
Ab2-BrdU IHC TL chemical treatment: 5-bromo-2'-deoxyuridine, chemical treatment: pharmaceutical Long-pec retina
IHC dctn1as309/s309 chemical treatment: 5-bromo-2'-deoxyuridine, chemical treatment: pharmaceutical Long-pec retina
Phenotype Details
Fish Conditions Stage Phenotype
dctn1as309/s309 chemical treatment: 5-bromo-2'-deoxyuridine, chemical treatment: pharmaceutical Long-pec retina layer formation disrupted, abnormal
dctn1as309/s309; rw021Tg standard conditions Day 5 retina layer formation disrupted, abnormal
Day 5 retinal ganglion cell increased amount, abnormal
Day 5 retinal ganglion cell mislocalised, abnormal
Day 5 retinal ganglion cell layer retinal ganglion cell increased amount, abnormal
dctn1as309/s309; s356tTg standard conditions Day 5 retina layer formation disrupted, abnormal
Day 5 retinal ganglion cell increased amount, abnormal
Day 5 retinal ganglion cell mislocalised, abnormal
Day 5 retinal ganglion cell layer retinal ganglion cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell