FIGURE

Fig. 6

ID

ZDB-FIG-080922-9

Publication

Guo et al., 1999 - Mutations in the zebrafish unmask shared regulatory pathways controlling the development of catecholaminergic neurons

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Fig. 6

no soul and soulless mutant embryos lack arch-associated CA cells. (A, B, and G–J) Lateral, (C–F) ventral, and (K and L) dorsal views of (A, C, E, G, I, and K) wildtype, (B, D, F, and H) no soul, and (J and L) soulless embryos. Embryos labeled with (A–D, I, and J) anti-TH antibody or (E–H) Alcian blue. (A and B) 2-day-old no soul embryos (B) showing the lack of aac but normal hypothalamic DA and LC neurons. (C and D) 2-day-old heterozygous no soul embryos (D) showing reduced size of aac clusters. (E–H) Cartilage derivatives of arch 2 are reduced (a small hyosymplectic may be present) and arches 3 and 4 are absent in a 5-dpf no soul fry. E and F are ventral and G and H are lateral views. (H) Elements of arch 1 are present, but displaced more ventrally (H). (I and J) 2-day-old soulless mutant embryos (J) showing absence of aac and LC neurons. (K and L) 96-h live soulless fry (J) showing abnormal positioning of the eyes. Abbreviations: aac, arch-associated CA cells; Hy DA, hypothalamic DA neurons; LC, locus coeruleus; P1–7, pharyngeal arches 1–7. Scale bar: 125 μm (A–D, I, J) and 200 μm (E, H, K, L).

Expression Data

Gene:	th
Fish:	WT foxi1^m809/m809 phox2a^m811/m811 phox2a^m812/m812 foxi1^m809/+ phox2a^m810
Anatomical Terms:	hypothalamus dopaminergic neuron locus coeruleus pharyngeal arch neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxi1^m809/m809 phox2a^m811/m811 phox2a^m812/m812 phox2a^m810
Observed In:	ceratohyal cartilage eye hyosymplectic cartilage hypothalamus dopaminergic neuron locus coeruleus locus coeruleus dopaminergic neuron mandibular arch skeleton Meckel's cartilage neuron pharyngeal arch pharyngeal arch 2 skeleton pharyngeal arch 3 skeleton pharyngeal arch 4 skeleton pharyngeal arch 5 skeleton pharyngeal arch 6 skeleton pharyngeal arch 7 skeleton swim bladder sympathetic nervous system sympathetic neuron telencephalon dopaminergic neuron whole organism
Stage Range:	Long-pec to Days 14-20

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 208, Guo, S., Wilson, S.W., Cooke, S., Chitnis, A.B., Driever, W., and Rosenthal, A., Mutations in the zebrafish unmask shared regulatory pathways controlling the development of catecholaminergic neurons, 473-487, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.