Fig. 6

Fig. 6 no soul and soulless mutant embryos lack arch-associated CA cells. (A, B, and G–J) Lateral, (C–F) ventral, and (K and L) dorsal views of (A, C, E, G, I, and K) wildtype, (B, D, F, and H) no soul, and (J and L) soulless embryos. Embryos labeled with (A–D, I, and J) anti-TH antibody or (E–H) Alcian blue. (A and B) 2-day-old no soul embryos (B) showing the lack of aac but normal hypothalamic DA and LC neurons. (C and D) 2-day-old heterozygous no soul embryos (D) showing reduced size of aac clusters. (E–H) Cartilage derivatives of arch 2 are reduced (a small hyosymplectic may be present) and arches 3 and 4 are absent in a 5-dpf no soul fry. E and F are ventral and G and H are lateral views. (H) Elements of arch 1 are present, but displaced more ventrally (H). (I and J) 2-day-old soulless mutant embryos (J) showing absence of aac and LC neurons. (K and L) 96-h live soulless fry (J) showing abnormal positioning of the eyes. Abbreviations: aac, arch-associated CA cells; Hy DA, hypothalamic DA neurons; LC, locus coeruleus; P1–7, pharyngeal arches 1–7. Scale bar: 125 μm (A–D, I, J) and 200 μm (E, H, K, L).