FIGURE

Fig. S1

ID

ZDB-FIG-080904-33

Publication

Emond et al., 2008 - Inhibition of protocadherin-alpha function results in neuronal death in the developing zebrafish

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Fig. S1

Antisense morpholino knockdown of pcdh2α results in neuronal cell death. A, TUNEL staining of a 24 hpf embryo injected with a 5 basepair mismatch morpholino showed low levels of cell death. B, A 24 hpf embryo injected with 4 ng of a splice-blocking morpholino directed against the pcdh2α constant exons resulted in a dramatic increase in cell death throughout the nervous system, similar to what was observed for the pcdh1α cluster. C, A dorsal view of the hindbrain of a 30 hpf embryo showed extensive cell death in the hindbrain. D, Injection of 4 ng of either MO2.1 (n = 546) or MO2.2 (n = 305) yielded the cell death phenotype in > 90% of embryos. Injection of 4 ng of a 5 basepair mismatch control morpholino did not result in cell death (n = 56).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-pcdh2a WT + MO2-pcdh2a
Knockdown Reagents:	MO1-pcdh2a MO2-pcdh2a
Observed In:	apoptotic process central nervous system neuron hindbrain neuron rhombomere neuron
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 321(1), Emond, M.R., and Jontes, J.D., Inhibition of protocadherin-alpha function results in neuronal death in the developing zebrafish, 175-187, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.