FIGURE

Fig. 3

ID

ZDB-FIG-080904-26

Publication

Emond et al., 2008 - Inhibition of protocadherin-alpha function results in neuronal death in the developing zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Pcdh1α intracellular domain is present in the nucleus. (A) Shown are dorsal views of the spinal cord from 24 hpf embryos; images are maximum intensity projections of two adjacent optical sections (1 μm spacing). The left panel shows DAPI labeling of cells. The middle panel shows the same field stained with antibody against Pcdh1αCD. The right panel is an overlay showing that Pcdh1α immunoreactivity is present in neuronal nuclei. (B) The leftmost panel is a maximum intensity projection of a spinal interneuron that has been labeled with a myc-tagged Pcdh1αCD. The myc-Pcdh1αCD fusion is distributed throughout the neuron. The middle two panels show single optical sections through the same neuron. The center-left panel shows staining of nuclei with DAPI and the center-right panel shows anti-myc immunolabeling. The myc-Pcdh1αCD is enriched in the nucleus. The overlay of the two fluorescence images is shown in the panel on the right. Scale bar = 10 μm.

Expression Data

Antibody:	Ab1-pcdh1a
Fish:	WT
Anatomical Terms:	neuron nucleus spinal cord
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 321(1), Emond, M.R., and Jontes, J.D., Inhibition of protocadherin-alpha function results in neuronal death in the developing zebrafish, 175-187, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.