Spatial-temporal Cre activity of Tg(zlmo2:Cre) transgenic line. A-C: The lmo2:LDL-EGFP+/-;wild-type embryo at 18 hpf. Expression of DsRed in the head vasculature (arrowhead) and ICM (arrow) in A; an amplified ventral view of head vasculature in B; and an amplified lateral view of ICM in C. D-F: The lmo2:LDL-EGFP+/-;wild-type embryo at 30 hpf. Expression of DsRed in the vessels in D (arrowhead and arrow); an amplified view of head vasculature in E; and an amplified lateral view of trunk vessels (arrow) and ISVs (star) in F. G-I: The lmo2:LDL-EGFP+/-;lmo2:Cre+/- double transgenic embryo at 18 hpf. Initiation of EGFP expression in the head vasculature and ICM with residual expression of DsRed fluorescence detected. J-L: The lmo2:LDL-EGFP+/-;lmo2:Cre+/- double transgenic embryo at 30 hpf. Initiation of EGFP expression in the vessels with residual expression of DsRed fluorescence detected. Lateral views in A,C,D-G, and I-L. All embryos are shown with heads to the left.
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