Fig. S2
- ID
- ZDB-FIG-080829-54
- Publication
- Kishi et al., 2008 - The identification of zebrafish mutants showing alterations in senescence-associated biomarkers
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Morpholino-induced knockdown of nrs and terf2 in zebrafish embryos generates phenocopies of the corresponding mutants. (A) nrs morphants show yolk-opaque phenotypes starting at 2.5 dpf (with PTU), which is an exact phenocopy of the nrs mutant embryos which also manifested an obvious yolk-opaque substance. Upon SA-β-gal staining at 3 dpf, an extremely high level of induction was detected in the nrs morphants, in an identical manner to the SA-β-gal levels observed in the nrs mutants, while the control-injected embryos did not show any significant SA-β-gal activity as well as opaque yolks. (B) terf2 morphants (3.5 dpf) show robust SA-β-gal induction having relatively moderate but quite a similar morphological phenotype to the terf2 allele mutant, terfahi1182/hi1182 which has a slightly weaker phenotype than terfahi3678/hi3678. |
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Stage Range: | Pec-fin to Protruding-mouth |