FIGURE

Fig. 2

ID

ZDB-FIG-080829-46

Publication

Kishi et al., 2008 - The identification of zebrafish mutants showing alterations in senescence-associated biomarkers

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Fig. 2

Induction of embryonic SA-β-gal activity by oxidative stress.
(A–D) Embryos treated with 500 μM BHP from 6 hpf to 6 dpf (B) have higher SA-β-gal activity than untreated embryos (A). Plots of the colorimetric quantitation of SA-β-gal staining show a near-linear increase of SA-β-gal activity with increased oxidative stress by BHP (C) or hydrogen peroxide (H₂O₂) (D). (E) Catalase overexpression can reduce SA-β-gal induction in embryos treated with moderate amounts of oxidative stress. Embryos injected with 300 pg of catalase mRNA at the one cell stage show significantly less SA-β-gal activity at 6 dpf than those injected with a control mRNA following incubation with 350 μM BHP from 6 hpf to 6 dpf. (F) Knockdown of endogenous catalase sensitizes embryos to oxidative stress-dependent SA-β-gal induction. Embryos injected with 8 ng of an antisense morpholino (MO) for catalase show a significant increase in SA-β-gal activity at 6 dpf when stressed with 350 μM BHP from 6 hpf to 6 dpf. *P<0.05 (Student t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cat
Condition:	chemical treatment: tert-butyl hydroperoxide
Knockdown Reagent:	MO1-cat
Observed In:	response to oxidative stress
Stage:	Day 6

Phenotype Detail

Acknowledgments

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