ZFIN Image: Kishi et al., 2008, Fig. S2

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Figure Caption

Fig. S2 Morpholino-induced knockdown of nrs and terf2 in zebrafish embryos generates phenocopies of the corresponding mutants. (A) nrs morphants show yolk-opaque phenotypes starting at 2.5 dpf (with PTU), which is an exact phenocopy of the nrs mutant embryos which also manifested an obvious yolk-opaque substance. Upon SA-β-gal staining at 3 dpf, an extremely high level of induction was detected in the nrs morphants, in an identical manner to the SA-β-gal levels observed in the nrs mutants, while the control-injected embryos did not show any significant SA-β-gal activity as well as opaque yolks. (B) terf2 morphants (3.5 dpf) show robust SA-β-gal induction having relatively moderate but quite a similar morphological phenotype to the terf2 allele mutant, terfa^{hi1182/hi1182} which has a slightly weaker phenotype than terfa^{hi3678/hi3678}.

Figure Data

Phenotype details

Acknowledgments

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