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ZFIN ID: ZDB-FIG-080828-86
Kim et al., 2008 - Notch-regulated oligodendrocyte specification from radial glia in the spinal cord of zebrafish embryos. Developmental dynamics : an official publication of the American Association of Anatomists   237(8):2081-2089 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec

Fig. 5 Conditional inhibition of Notch signaling before OPC specification produces excess secondary motor neurons at the expense of OPCs. All images show transverse sections of spinal cord, dorsal to top. A-D: Control embryos. E-H: Tg(hsp70l:dnSu(H)myc) embryos heat-shocked at 30 hours postfertilization (hpf). Inhibition of Notch signaling before OPC specification blocks formation of Sox10+ OPCs (A,E,I) and produces excess secondary motor neurons (B,F,J). Arrows mark OPCs and secondary motor neurons and the small, dashed circle marks the area around the central canal, which is not occupied by motor neurons, in control embryos. The medial portion of the spinal cord, which does not have Hu+ neurons in control embryos (dashed circle, C) is filled with neurons in Notch-inhibited embryos (G). Pulse labeling with bromodeoxyuridine (BrdU, red) reveals that more dividing spinal cord cells adopt Isl+ motor neuron fates in Notch-inhibited embryos relative to controls (D,H,K). The quantitative data were obtained from sections of six control and six heat-shocked embryos for each experiment. Error bars represent SEM. Scale bar = 20 μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
sox10 Ab1-sox10 AB standard conditions Long-pec glioblast (sensu Vertebrata) IHC
Ab1-sox10 Long-pec spinal cord IHC
Ab1-sox10 vu21Tg heat shock Long-pec glioblast (sensu Vertebrata) IHC
Ab1-sox10 Long-pec spinal cord IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-G3G4 IHC AB chemical treatment: 5-bromo-2'-deoxyuridine Prim-25 motor neuron
IHC Prim-25 spinal cord
IHC vu21Tg heat shock, chemical treatment: 5-bromo-2'-deoxyuridine Prim-25 motor neuron
IHC Prim-25 spinal cord
Ab1-elavl IHC AB standard conditions Prim-25 neuron
IHC Prim-25 spinal cord
IHC vu21Tg heat shock Prim-25 neuron
IHC Prim-25 spinal cord
Ab2-isl IHC AB standard conditions Prim-25 secondary motor neuron
IHC Prim-25 spinal cord
IHC AB chemical treatment: 5-bromo-2'-deoxyuridine Prim-25 motor neuron
IHC Prim-25 spinal cord
IHC vu21Tg heat shock, chemical treatment: 5-bromo-2'-deoxyuridine Prim-25 motor neuron
IHC Prim-25 spinal cord
IHC vu21Tg heat shock Prim-25 secondary motor neuron
IHC Prim-25 spinal cord
zn-8 IHC AB standard conditions Prim-25 secondary motor neuron
IHC Prim-25 spinal cord
IHC vu21Tg heat shock Prim-25 secondary motor neuron
IHC Prim-25 spinal cord
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.