FIGURE

Fig. 5

ID

ZDB-FIG-080828-44

Publication

Lecaudey et al., 2008 - Dynamic Fgf signaling couples morphogenesis and migration in the zebrafish lateral line primordium

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Fig. 5

FGF is required for rosette assembly, a prerequisite for primordium migration. Primordia of cxcr4b mutant embryos treated with DMSO (A) or SU5402 (B), showing the complete disappearance of rosettes after blocking the FGF pathway for 6 hours. (C,D) Time-lapse analysis and corresponding kymograph showing the progressive melting of rosettes after treatment with 80 μM SU5402. The migration speed is normal (70 μm/hour) and remains constant during this phase. Scale bar: 50 μm. (E,F) Time-lapse analysis and corresponding kymograph of a washout experiment showing that five rosettes (red arrows in E,F) simultaneously reassemble before migration resumes. Three phases can be distinguished on the kymograph: (1) uncoordinated migration, (2) rosette reassembly, and (3) migration recovery. (G,H) Expression of Fgf ligands in cldnb:gfp embryos treated for 6 hours with SU5402. Lower panels are transmission images of NBT/BCIP stainings. fgf3 (G) and fgf10 (H) are strongly expressed throughout the primordium when Fgfr is blocked. Scale bars: 20 μm (unless stated otherwise).

Expression Data

Genes:	EGFP fgf3 fgf10a
Fish:	zf106Tg cxcr4b^{t26035/t26035}; zf106Tg
Condition:	chemical treatment: SU5402
Anatomical Term:	posterior lateral line primordium
Stage Range:	Prim-15 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cxcr4b^{t26035/t26035}; zf106Tg
Condition:	chemical treatment: SU5402
Observed In:	posterior lateral line neuromast development
Stage Range:	Prim-15 to Prim-25

Phenotype Detail

Acknowledgments

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