FIGURE

Fig. 8

ID

ZDB-FIG-080702-32

Publication

Lee et al., 2008 - Zebrafish blowout provides genetic evidence for Patched1-mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye

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Fig. 8

atp6v0b morpholinos do not phenocopy blowout. (A) The atp6v0b predicted genomic organization. atp6v0b MO targets the exon1/intron1 splice junction and leads to the inclusion of a 705 bp intron1 and the introduction of a premature stop codon. (B) RT-PCR validation of splice blocking efficacy of the atp6v0b MO. (C) Control injected and (D) atp6v0b morphant (13 ng) at 5 dpf. Morphants display oculocutaneous albinism and retinal degeneration but show no signs of colobomas even at the highest doses of MO injection.

Expression Data

Gene:	atp6v0b
Fish:	AB AB + MO1-atp6v0b
Knockdown Reagent:	MO1-atp6v0b
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-atp6v0b
Knockdown Reagent:	MO1-atp6v0b
Observed In:	eye integument retina
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 319(1), Lee, J., Willer, J.R., Willer, G.B., Smith, K., Gregg, R.G., and Gross, J.M., Zebrafish blowout provides genetic evidence for Patched1-mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye, 10-22, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.