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ZFIN ID: ZDB-FIG-080702-27
Lee et al., 2008 - Zebrafish blowout provides genetic evidence for Patched1-mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye. Developmental Biology   319(1):10-22 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 2 Retinal development is largely normal in blowout. Immunohistochemical analysis of retinal ganglion cells (RGCs) via zn8 staining (A, D), rod photoreceptors via zpr3 staining (B, E) and red/green double cones via zpr1 staining (C, F) in transverse retinal cryosections. All retinal cell types are present and are properly distributed in blw mutants, and retinal cell numbers are also similar between wild-type and blw mutant eyes. RGC organization is affected in blw mutants where RGC axons do not assemble into a tight bundle as they exit the eye (arrow in panel D). Photoreceptor outer segments are present in the retinal territories that are not contained within the eye cup (arrows in panels E, F). Antibody stains are red and nuclei are counterstained green with Sytox Green. Dorsal is up in all images.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-8 IHC WT standard conditions Day 5 retinal ganglion cell
IHC ptch2tc294z/tc294z standard conditions Day 5 retinal ganglion cell
zpr-1 IHC WT standard conditions Day 5 retinal cone cell
IHC ptch2tc294z/tc294z standard conditions Day 5 retinal cone cell
zpr-3 IHC WT standard conditions Day 5 retinal rod cell
IHC ptch2tc294z/tc294z standard conditions Day 5 retinal rod cell
Phenotype Details
Fish Conditions Stage Phenotype
ptch2tc294z/tc294z standard conditions Day 5 optic fissure closure incomplete, abnormal
Day 5 photoreceptor outer segment layer protruding, abnormal
Day 5 retina protruding, abnormal
Day 5 retinal ganglion cell axon disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(1), Lee, J., Willer, J.R., Willer, G.B., Smith, K., Gregg, R.G., and Gross, J.M., Zebrafish blowout provides genetic evidence for Patched1-mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye, 10-22, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.