FIGURE

Fig. S1

ID

ZDB-FIG-080604-45

Publication

Chi et al., 2008 - Genetic and Physiologic Dissection of the Vertebrate Cardiac Conduction System

Other Figures

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Fig. S1

Comparison of Calcium-Green–Injected Embryos versus Stable Tg(cmlc2:gCaMP)^s878 Embryos
(A, E, I, M, Q) Epifluorescence micrographs of calcium-green-injected live zebrafish embryos at 24, 48, 72, 96, and 120 hpf. Note ubiquitous and strong fluorescence throughout most of the embryo.

(B, F, J, N, R) Higher magnification epifluorescence micrographs of calcium-green-injected embryos focusing on the hearts at 24, 48, 72, 96, and 120 hpf. Weaker calcium-green fluorescence is observed as the embryos develop.

(C, G, K, O, S) Epifluorescence micrographs of Tg(cmlc2:gCaMP)^s878 live embryos at 24, 48, 72, 96, and 120 hpf. Specific gCaMP fluorescence is detected only in hearts. Autofluorescence is detected in the yolk.

(D, H, L, P, T) Higher magnification epifluorescence micrographs of Tg(cmlc2:gCaMP)^s878 embryos focusing on the hearts at 24, 48, 72, 96, and 120 hpf. Autofluorescence from yolk does not interfere with imaging the hearts.
At, atrium; V, ventricle; HT, heart tube.

Expression Data

Gene:	EGFP
Fish:	s878Tg
Anatomical Terms:	heart primitive heart tube
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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