ZFIN ID: ZDB-FIG-080604-16
Reichenbach et al., 2008 - Endoderm-derived Sonic hedgehog and mesoderm Hand2 expression are required for enteric nervous system development in zebrafish. Developmental Biology   318(1):52-64 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Pec-fin

Fig. 4 Hedgehog pathway mutants lack enteric ganglia. (A–I) Dorsal views, anterior to the left. Enteric ganglia colonizing the gut express phox2b in wild type (A, black arrows) while syu-/- (B) and smu-/- (C) embryos show no phox2b expression around the gut tube. (D) Expression of ret in 36 hpf wild type embryos in migrating enteric precursors (black arrows). In syu-/- (E) and smu-/- (F) no ret expressing migrating cells can be detected. Wild type embryos at 36 hpf show crestin expression in vagal neural crest cells migrating to the anterior gut (G, black arrows). syu-/- (H) and smu-/- (I) embryos lack crestin expressing vagal neural crest cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
crestin WT standard conditions Prim-15 presumptive enteric nervous system ISH
shhatq252/tq252 standard conditions Prim-15 Not Detected presumptive enteric nervous system ISH
smounspecified/unspecified standard conditions Prim-15 Not Detected presumptive enteric nervous system ISH
phox2bb WT standard conditions Pec-fin enteric nervous system ISH
shhatq252/tq252 standard conditions Pec-fin Not Detected enteric nervous system ISH
smounspecified/unspecified standard conditions Pec-fin Not Detected enteric nervous system ISH
ret WT standard conditions Prim-15 presumptive enteric nervous system ISH
shhatq252/tq252 standard conditions Prim-15 Not Detected presumptive enteric nervous system ISH
smounspecified/unspecified standard conditions Prim-15 Not Detected presumptive enteric nervous system ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 318(1), Reichenbach, B., Delalande, J.M., Kolmogorova, E., Prier, A., Nguyen, T., Smith, C.M., Holzschuh, J., and Shepherd, I.T., Endoderm-derived Sonic hedgehog and mesoderm Hand2 expression are required for enteric nervous system development in zebrafish, 52-64, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.