FIGURE

Fig. 2

ID
ZDB-FIG-080516-44
Publication
Minchin et al., 2008 - Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Pax3 knockdown blocks terminal differentiation of trunk xanthophores whilst other pigment cell types differentiate. Live control (A, C, E, G, I) or pax3 MO-injected (B, D, F, H, J) zebrafish larvae showing yellow xanthophore pigmentation in brightfield images (A–D), stained with methylene blue to reveal pterinosomes (E–H) or in darkfield to reveal iridophores (I, J). Lateral views (A–F, I, J) are anterior to left and dorsal to top. Dorsal views of head region (G, H) are anterior to left and lateral to top. (A–D) At 5 dpf, yellow xanthophore pigmentation is visible on the dorsal head and trunk (arrowheads). Embryonic melanophores are arranged in stripes (arrows). In pax3 morphants, no yellow xanthophore pigmentation is visible in the trunk and is reduced in the dorsal head (B, arrowhead), whereas embryonic melanophore stripes are still present and located correctly (B, D, arrows). (E–H) At 5 dpf, blue pterinosomes (arrowheads) coincide with xanthophore locations in dorsal and posterior regions overlying epaxial and hypaxial somite in the trunk (E) and above the eye in the head (G, white arrowhead). In pax3 morphants, pterinosome stain is absent from the trunk (F) and reduced in the head (H, arrowhead). (I, J) At 6 dpf, shiny iridophore pigment cells (white arrows) have begun to differentiate and are located at the dorsal and ventral larval melanophore stripes (I). Overlying xanthophores give the iridophores a yellow tint. In pax3 morphants, iridophores differentiate in both dorsal and ventral positions but appear white due to the lack of overlying xanthophores (J). (K) Severity of methylene blue-stained xanthophore phenotype in trunk is dependent on dose of pax3 MO. Overall reduction in trunk xanthophores (reduced) was distinguished from complete absence in most of the trunk (severely reduced). Number of embryos analysed (n) are given above columns.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Day 5 to Day 6

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 317(2), Minchin, J.E., and Hughes, S.M., Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest, 508-522, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.