ZFIN ID: ZDB-FIG-080515-8
Winkler et al., 2001 - Zebrafish mdk2, a novel secreted midkine, participates in posterior neurogenesis. Developmental Biology   229(1):102-118 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage Range: Shield to Prim-5

Fig. 6 mdk2 expression is independent of FGF8 and nodal-related1 signaling, inhibited by BMP signaling, and upregulated by low doses of exogenously applied RA. (A, B) Lateral views of mdk2 expression in 24-hpf wild-type (wt, A) and acerebellar (ace, B) mutant embryos. mdk2 expression at the MHB (arrow) is absent due to lack of cerebellar structure. Otherwise expression appears unaffected (arrowheads). ey, eye. (C, D) Dorsal views of mdk2 (arrowhead) and gsc (arrow) expression in wt (C) and cyclops (cyc, D) mutant embryos at 90% epiboly. gsc is repressed in cyc embryos, but mdk2 expression appears unaffected. (E–P) Lateral views, dorsal to right. (E, F) Expression of eve1 (arrowhead) and mdk2 (arrow) in wt and chordino (chd) mutant embryos at 70% epiboly. eve1 expression is expanded in chd embryos, but mdk2 is repressed (arrow in F compared to E). (G–J) Expression of eve1 (arrowhead) and mdk2 (arrow) in wt (G, I), snailhouse (snh, H), and swirl (swr, J) mutant embryos at 80% epiboly. While eve1 is repressed in snh and swr, mdk2 expression is ventrally expanded. (K–P) Expression levels of mdk2 (arrowhead) are dependent on the dose of exogenously applied RA. (K–N) Dose response of mdk2 expression in 70% epiboly embryos. RA applied at 10-7 M reduces mdk2 expression (L), whereas lower doses (M, N) increase expression when compared to untreated embryos (K). (O) Expression of mdk2 in an untreated embryo at 70% epiboly. (P) Complete repression of mdk2 expression by treatment with RA at a dose of 10-6 M.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mdkb WT standard conditions Shield anatomical structure ISH
75%-epiboly anatomical structure ISH
Prim-5 cerebellum ISH
Prim-5 diencephalon ISH
Prim-5 hindbrain ISH
Prim-5 spinal cord ISH
WT chemical treatment: all-trans-retinoic acid Shield anatomical structure ISH
bmp7aty68a/ty68a standard conditions 75%-epiboly anatomical structure ISH
chrdtt250/tt250 standard conditions Shield Not Detected anatomical structure ISH
fgf8ati282a/ti282a standard conditions Prim-5 Not Detected cerebellum ISH
Prim-5 diencephalon ISH
Prim-5 hindbrain ISH
Prim-5 spinal cord ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 229(1), Winkler, C. and Moon, R.T., Zebrafish mdk2, a novel secreted midkine, participates in posterior neurogenesis, 102-118, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.