ZFIN ID: ZDB-FIG-080514-54
Willot et al., 2002 - Cooperative action of ADMP- and BMP-mediated pathways in regulating cell fates in the zebrafish gastrula. Developmental Biology   241(1):59-78 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 10-13 somites to Prim-5

Fig. 9 Overexpression of admp, tr-admp, or admp-morpholino in din mutants. tr-admp RNA (600 pg), admp RNA (600 pg), or admp-morpholino (Mo) (250 μM) was injected at the one-cell stage into the progeny of a din-/- x din-/+ cross. (A–D) Phenotypes were observed live after 1 day of development or (E–I) after in situ hybridization at the 10-somite stage; fkd6, her5, and gata1 in dark blue, hgg1, krox20, and ntl in red. (A) Uninjected embryo, din-/- phenotype. (B) tr-admp-injected din-/- embryo, partially rescued. (C) admp injected din-/- embryo, enhanced phenotype. (D) Mo-injected din-/- embryo, partially rescued. (A–D) Arrowhead, compare the size of the eye; arrows, ventral tail fin and vein, lost in (B); and amount of blood cells slightly reduced in (B) and (D). (E) Uninjected, -/+ or +/+ embryo. (F) Uninjected embryo, din-/- phenotype. gata1 expression domain is enlarged (arrowhead) (G) admp injected din-/- embryo, enhanced phenotype. Note the absence of posterior notochord (arrow) and the enlarged gata1 expression domain (arrowhead). (H) Mo-injected din-/- embryo, partially rescued. The notochord is enlarged (arrow); compare with (A) and (B). (I) tr-admp-injected din-/- embryo, partially rescued. The notochord is enlarged (arrow).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
chrdtt250/tt250 standard conditions 10-13 somites whole organism wholly ventralized, abnormal
Prim-5 whole organism wholly ventralized, abnormal
chrdtt250/tt250 + MO1-admp standard conditions 10-13 somites whole organism wholly ventralized, abnormal
Prim-5 whole organism wholly ventralized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 241(1), Willot, V., Mathieu, J., Lu, Y., Schmid, B., Sidi, S., Yan, Y.-L., Postlethwait, J.H., Mullins, M., Rosa, F., and Peyriéras, N., Cooperative action of ADMP- and BMP-mediated pathways in regulating cell fates in the zebrafish gastrula, 59-78, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.