FIGURE

Fig. 10

ID
ZDB-FIG-080505-33
Publication
Parvin et al., 2008 - Autoregulatory loop and retinoic acid repression regulate pou2/pou5f1 gene expression in the zebrafish embryonic brain
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Fig. 10

Binding of the DR2-type retinoic acid-responsive element (RARE) with the RAR/RXR complex. A: Schemes of the GFP-2.2 constructs that are intact or possesses a disrupted RARE (GFP-2.2ΔRARE). B: Sequences of the three oligos, Rf-RARE, P2-RARE, and P2-RAREm, are aligned. Sequences corresponding to the RARE consensus, which is shown at the bottom, are underlined. The bases substituted in P2-RAREm compared with the original oligo (P2-RARE) are shown with lower-case letters. C: Electrophoretic gel mobility shift assay (EMSA) showing specific binding of RAR/RXR with P2-RARE. Both P2-RARE and Rf-RARE formed complexes with RAR/RXR, which were competed out by a 100-fold molar excess of P2-RARE and/or Rf-RARE, but not by P2-RAREm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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