ZFIN ID: ZDB-FIG-080501-3
Kleinjan et al., 2008 - Subfunctionalization of Duplicated Zebrafish pax6 Genes by cis-Regulatory Divergence. PLoS Genetics   4(2):e29 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 3 Analysis of pax6b Function in Zebrafish, with the Aim of Validating the Effect of the pax6b L244P Missense Mutation in sri Fish
(A–D) Phenotype elicited using pax6b morpholinos: (A) Uninjected wild type fish at 48 hpf; fish injected with the same amount of a control scrambled morpholino also produced no effect.
(B) Substantially reduced eye size and generally delayed development are observed by 48 hpf in response to injection of either of the two pax6b morpholinos.
(C and D) At 32 hpf reduction in eye size is observed (white dotted outlines) in otherwise normal looking embryos.
(E) The effects of injecting capped mRNA at levels that generally rescue the phenotype illustrating the spectrum of phenotypes to bilateral anophthalmia, observed when pax6b capped mRNA is injected at levels generally used for phenotype rescue experiments in zebrafish.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax6a WT standard conditions Prim-15 eye ISH
Prim-15 hindbrain ISH
WT + MO2-pax6b standard conditions Prim-15 eye ISH
Prim-15 hindbrain ISH
WT + MO3-pax6b standard conditions Prim-15 eye ISH
Prim-15 hindbrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-pax6b standard conditions Prim-15 eye decreased size, abnormal
Prim-15 eye development disrupted, abnormal
Long-pec embryo development disrupted, abnormal
Long-pec embryo development paedomorphic growth, abnormal
Long-pec eye decreased size, abnormal
Long-pec eye development disrupted, abnormal
WT + MO3-pax6b standard conditions Prim-15 eye decreased size, abnormal
Long-pec embryo development disrupted, abnormal
Long-pec embryo development paedomorphic growth, abnormal
Long-pec eye decreased size, abnormal
Long-pec eye development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.