FIGURE

Fig. 5

ID

ZDB-FIG-080422-9

Publication

Chung et al., 2008 - Intra-endodermal interactions are required for pancreatic beta cell induction

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Smoothened Function Is Required in the Endoderm for the Induction of Dorsal Pancreatic β Cells

(A) Schematic diagram of the cell transplantation protocol. cas-overexpressing wild-type or smo mutant Tg(ins:GFP) donor cells were transplanted into cas MO-injected wild-type hosts.

(B–E) Dorsal views of the cas MO-injected hosts with wild-type (B and D) and smo mutant (C and E) donor cells at 24 hpf. Donor cells are labeled with rhodamine dextran (red). All the endodermal cells of the hosts are replaced by donor cells. (B and D) Wild-type-like Tg(ins:GFP) expression in the endoderm derived from wild-type donor cells (95%, n = 74/78). (C and E) Tg(ins:GFP)-expressing cells are completely absent ([C], 92%, n = 23/25) or amount to one or two cells ([E], arrow; 8%, n = 2/25) when smo mutant donor cells form the endoderm in cas MO-injected wild-type hosts.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 14(4), Chung, W.S., and Stainier, D.Y., Intra-endodermal interactions are required for pancreatic beta cell induction, 582-593, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell