ZFIN Image: Chung et al., 2008, Fig. 5

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Figure Caption

Fig. 5 Smoothened Function Is Required in the Endoderm for the Induction of Dorsal Pancreatic β Cells

(A) Schematic diagram of the cell transplantation protocol. cas-overexpressing wild-type or smo mutant Tg(ins:GFP) donor cells were transplanted into cas MO-injected wild-type hosts.

(B–E) Dorsal views of the cas MO-injected hosts with wild-type (B and D) and smo mutant (C and E) donor cells at 24 hpf. Donor cells are labeled with rhodamine dextran (red). All the endodermal cells of the hosts are replaced by donor cells. (B and D) Wild-type-like Tg(ins:GFP) expression in the endoderm derived from wild-type donor cells (95%, n = 74/78). (C and E) Tg(ins:GFP)-expressing cells are completely absent ([C], 92%, n = 23/25) or amount to one or two cells ([E], arrow; 8%, n = 2/25) when smo mutant donor cells form the endoderm in cas MO-injected wild-type hosts.

Acknowledgments

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Reprinted from Developmental Cell, 14(4), Chung, W.S., and Stainier, D.Y., Intra-endodermal interactions are required for pancreatic beta cell induction, 582-593, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell