FIGURE

Fig. 7

ID

ZDB-FIG-080411-42

Publication

Yamaguchi et al., 2008 - Mutation of DNA primase causes extensive apoptosis of retinal neurons through the activation of DNA damage checkpoint and tumor suppressor p53

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Fig. 7

piy-mediated apoptosis depends on the ATM-Chk2-p53 pathway. (A) Quantitative PCR analysis of p53 mRNA in wild-type and piy mutant zebrafish embryos. There is no difference in the amplification of a control gene, ef1α (lower panel), from wild-type and the piy mutant cDNA pools. By contrast, the p53 gene is amplified from the piy mutant cDNA pool by a smaller number of PCR cycles than from the wild-type cDNA pool (upper panel), suggesting that p53 mRNA is more abundant in the piy mutant embryos than in wild-type embryos. (B,C) Plastic sections of piy mutant retinas injected without (B) and with (C) MO-Chk2. (D,E) Plastic sections of piy mutant retinas injected without (D) and with (E) MO-p53. (F-H) Treatment of the piy mutant embryos with DMSO (F), KU55933 (G) or CGK733 (H).

Expression Data

Antibodies:	zn-5 zpr-1
Fish:	WT prim1^rw255/rw255 (RW) prim1^rw255/rw255 + MO4-tp53 (RW)
Knockdown Reagent:	MO4-tp53
Anatomical Terms:	retinal cone cell retinal ganglion cell
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	prim1^rw255/rw255 (RW) prim1^rw255/rw255 + MO1-chek2 (RW) prim1^rw255/rw255 + MO4-tp53 (RW)
Condition:	chemical treatment: protein kinase inhibitor
Knockdown Reagents:	MO1-chek2 MO4-tp53
Observed In:	eye retina retinal cone cell retinal ganglion cell retinal neural layer apoptotic process
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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